Modification of transmembrane and GPI-anchored proteins on living cells by efficient protein trans-splicing using the Npu DnaE intein.

نویسندگان

  • Tulika Dhar
  • Henning D Mootz
چکیده

The naturally split Npu DnaE intein can be used for ligation of an exogenous polypeptide to membrane proteins on living cells. No reducing agents or other factors are required. The approach is rapid and virtually traceless, because the intein removes itself during the reaction.

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عنوان ژورنال:
  • Chemical communications

دوره 47 11  شماره 

صفحات  -

تاریخ انتشار 2011